Food engineering and bioengineering
SUN Zhuo, ZHANG Song, SU Le, YUE Qiulin, SUN Xin, LI Kunlun, ZHAO Lin
To investigate the immunomodulatory effects of porcine skin collagen peptides on RAW264.7 cells,this study prepared Porcine skin collagen peptides via enzymatic hydrolysis and analyzed their amino acid composition and molecular weight distribution.The effects of porcine collagen peptides on RAW264.7 cells were systematically evaluated using a range of methods,including the MTT assay,neutral red phagocytosis assay,cell morphological observation,cytokine content measurement,and real-time quantitative PCR.These analyses assessed cell proliferation activity,phagocytic function,morphological changes,the production levels of NO,TNF-α,IL-2,and IL-12,as well as the mRNA expression of related immune factors(iNOS,TNF-α,IL-2,and IL-12).The results demonstrated that Porcine skin collagen peptides are primarily composed of glycine,proline,alanine,glutamic acid,aspartic acid,and arginine,with molecular weights mainly distributed between 500 and 5 000 Da.Porcine skin collagen peptides significantly promoted RAW264.7 cell proliferation and phagocytosis,induced morphological changes,enhanced NO,TNF-α,IL-2,and IL-12 production,and upregulated the gene expression of iNOS,TNF-α,IL-2,and IL-12.In conclusion,Porcine skin collagen peptides exhibit potential in activating macrophages and enhancing non-specific immune functions.